Regulatory

Part:BBa_K2865000:Design

Designed by:   Group: iGEM18_SMMU-China   (2018-09-22)


BNP promoter, heart failure inducible


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 242


Design Notes

In vivo studies of BNP stress-responsive regions revealed that -408/+100 bp hBNP promoter fragment is able to increase luciferase reporter activity after acute MI and AngⅡ infusion.


Source

approximal region (-408/+100bp) of BNP promoter

References